4°C for 3 months; −20°C for 2 years.
ProScale Protein Marker 250 (10–250 kDa)
Size: 500μL：250 μL*2/250 0μL：250 μL*10
Product Description
The ProScale Protein Marker 250 is a ready-to-use three-color protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 250 kDa (10 to 230 kDa in Bis-Tris (MOPS) buffer and 9.8 to 230 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (red band at 70 kDa and green band at 25 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). This marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose), and for approximating the size of proteins.
Contents
Approximately 0.1–0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2 mM dithiothreitol, 3.6 M urea, and 15% (v/v) glycerol).
Storage
Notice
Ready to use: Do NOT heat, dilute, or add reducing agents before loading.
Reference bands: 70 kDa (red), 25 kDa (green).
Loading volume:
3–5 µL per well for mini gel
1.5–2.5 µL per well for Western transfer
6–10 µL per well for large gel
- Type of membranes: PVDF, nylon, or nitrocellulose.
Guide for Molecular Weight Estimation
Migration patterns and approximate molecular weights (kDa) of ProScale Protein Marker 250 in:
| Band | Color | Tris-Glycine (kDa) | Bis-Tris (MOPS) (kDa) | Bis-Tris (MES) (kDa) |
|---|---|---|---|---|
| 1 | Blue | 250 | 230 | 230 |
| 2 | Blue | 150 | 140 | 140 |
| 3 | Blue | 100 | 98 | 98 |
| 4 | Red | 70 | 63 | 63 |
| 5 | Blue | 50 | 49 | 49 |
| 6 | Blue | 40 | 39 | 38 |
| 7 | Blue | 35 | 34 | 33 |
| 8 | Green | 25 | 25 | 24 |
| 9 | Blue | 20 | 20 | 21 |
| 10 | Blue | 15 | 15 | 14 |
| 11 | Blue | 10 | 10 | 9.8 |
